Abstract
ACID dyes at high alkaline pH (8–10.5) have been found by us to stain aortic elastin in a variety of different mammals (see also Spicer and Lillie1). Bromphenol blue is the best dye for the purpose, as it is less easily removed by subsequent dehydrating alcohols. Our attention has been directed to the effect of somatic age on this elastin stain. If one compares formol-fixed aortic elastin of a human infant with the same structure in a 70-year-old, bromphenol blue stains the elastin of the aged aorta less intensely than the juvenile one.
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References
Spicer, S. S., and Lillie, R. D., Stain Technol., 36, 365 (1961).
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MENZIES, D., ROBERTS, J. Effect of Age on the Acidophilia of Aortic Elastin. Nature 198, 1006–1007 (1963). https://doi.org/10.1038/1981006a0
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DOI: https://doi.org/10.1038/1981006a0
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