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Replacement of Thymidylic Acid by Deoxyuridylic Acid in the Deoxyribonucleic Acid of a Transducing Phage for Bacillus subtilis


THE findings that the thermal denaturation temperature (Tm) of DNA from a wide variety of bacterial, plant and animal sources as well as the buoyant density in a cæsium chloride gradient are linearly related to the guanine plus cytosine (G plus C) base composition1–2 have made it possible to search for the existence of DNA samples which would show an anomalous behaviour with respect to these two parameters. Native DNA preparations which show no correlation between their buoyant density and Tm would be expected to have components other than those usually encountered in DNA3; this situation might arise with a high degree of probability in the DNA isolated from bacteriophages or viruses, which because of their specialized behaviour would be expected to possess distinctive structural features.

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TAKAHASHI, I., MARMUR, J. Replacement of Thymidylic Acid by Deoxyuridylic Acid in the Deoxyribonucleic Acid of a Transducing Phage for Bacillus subtilis. Nature 197, 794–795 (1963).

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