Abstract
o-Nitrophenyl β-D-galactoside (ONPG), which was introduced by Lederberg1as a chromogenic substrate for β-galactosidases, has found widespread application in enzyme investigations. We have recently reported both a method for preparing partially acetylated glucose derivatives2and the action of esterases3on these compounds. The synthesis was adapted to a galactose derivative—6-O-acetyl o-nitrophenyl β-D-galactoside (I)—with the view of examining both the specificity of certain β-galactosidases and esterases and the interdependence, if any, of these two enzyme groups.
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References
Lederberg, J., J. Bact., 60, 381 (1950).
Frohwein, Y. Z., and Leibowitz, J., Nature, 186, 153 (1960).
Frohwein, Y. Z., and Leibowitz, J., Enzymologia, 23, 202, 208 (1961).
Seidman, M., and Link, K. P., J. Amer. Chem. Soc., 72, 4324 (1950).
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FROHWEIN, Y. 6-O-Acetyl o-Nitrophenyl β-D-Galactoside. Nature 196, 775–776 (1962). https://doi.org/10.1038/196775a0
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DOI: https://doi.org/10.1038/196775a0
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