6-O-Acetyl o-Nitrophenyl β-D-Galactoside


o-Nitrophenyl β-D-galactoside (ONPG), which was introduced by Lederberg1as a chromogenic substrate for β-galactosidases, has found widespread application in enzyme investigations. We have recently reported both a method for preparing partially acetylated glucose derivatives2and the action of esterases3on these compounds. The synthesis was adapted to a galactose derivative—6-O-acetyl o-nitrophenyl β-D-galactoside (I)—with the view of examining both the specificity of certain β-galactosidases and esterases and the interdependence, if any, of these two enzyme groups.

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  1. 1

    Lederberg, J., J. Bact., 60, 381 (1950).

  2. 2

    Frohwein, Y. Z., and Leibowitz, J., Nature, 186, 153 (1960).

  3. 3

    Frohwein, Y. Z., and Leibowitz, J., Enzymologia, 23, 202, 208 (1961).

  4. 4

    Seidman, M., and Link, K. P., J. Amer. Chem. Soc., 72, 4324 (1950).

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FROHWEIN, Y. 6-O-Acetyl o-Nitrophenyl β-D-Galactoside. Nature 196, 775–776 (1962) doi:10.1038/196775a0

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