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Malic Enzyme Activity in Apple Fruit


THE capacity of apple fruit to decarboxylate malic acid oxidatively (the malate effect) is evident early in apple fruit ontogeny1, as well as in the stages characterized by the climacteric and post-climacteric phases of fruit respiration2. The stoichiometric characteristics of the malate decarboxylation reaction1 and the symmetrical distribution of carbon-14 in malic acid resulting from 14CO2 fixation in apple fruit3 suggested the malic enzyme may function in both reactions. This investigation was initiated to determine if malic enzyme is responsible for malate decarboxylation and carbon dioxide fixation into malic acid in apple fruit.

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  1. Flood, A. E., Hulme, A. C., and Wooltorton, L. S. C., J. Exp. Bot., 11, 316 (1960).

    Article  CAS  Google Scholar 

  2. Neal, G. E., and Hulme, A. C., J. Exp. Bot., 9, 142 (1958).

    Article  CAS  Google Scholar 

  3. Allentoff, N., Phillips, W. R., and Johnston, F.B., J. Sci. Food Agric., 5, 234 (1954).

    Article  CAS  Google Scholar 

  4. Lieberman, M., Plant Physiol., 35, 796 (1960).

    Article  CAS  Google Scholar 

  5. Hageman, R. H., and Waygood, E. R., Plant Physiol., 34, 396 (1959).

    Article  CAS  Google Scholar 

  6. Warburg, O., and Christian, W., Biochem. Z., 310, 385 (1941).

    Google Scholar 

  7. Ochoa, S., Mehler, A. H., and Kornberg, A., J. Biol. Chem., 174, 979 (1948).

    CAS  Google Scholar 

  8. Rutter, W. J., and Lardy, H. A., J. Biol. Chem., 233, 374 (1958).

    CAS  PubMed  Google Scholar 

  9. Hulme, A. C., J. Exp. Bot., 5, 159 (1954).

  10. Harary, I., Korey, S. R., and Ochoa, S., J. Biol. Chem., 203, 595 (1953).

    CAS  PubMed  Google Scholar 

  11. Hulme, A. C., and Neal, G. E., Nature, 179, 1192 (1957).

    Article  ADS  CAS  Google Scholar 

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DILLEY, D. Malic Enzyme Activity in Apple Fruit. Nature 196, 387–388 (1962).

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