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Use of Ferritin-Conjugated Antibody to identify Nephrotoxic Sera in Renal Tissue by Electron Microscopy

Abstract

EXPERIMENTAL acute glomerulonephritis is readily produced in rats by the injection of rabbit antibody into rat kidney1. It has been found by radioautography that labelled nephrotoxic antibody was present in glomeruli2. Investigations with the fluorescent antibody technique afterwards showed the deposition of nephrotoxic globulin in what appeared to be basement membranes3,4; but lack of resolution made precise localization difficult.

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ANDRES, G., MORGAN, C., HSU, K. et al. Use of Ferritin-Conjugated Antibody to identify Nephrotoxic Sera in Renal Tissue by Electron Microscopy. Nature 194, 590–591 (1962). https://doi.org/10.1038/194590a0

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  • DOI: https://doi.org/10.1038/194590a0

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