Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Vitamin K1 in Chloroplasts

Abstract

THERE has been considerable speculation concerning the role of vitamin K in photosynthesis. Arnon1 has shown that several water-soluble naphthoquinones stimulate photosynthetic phosphorylation, although vitamin K1 is inactive as a stimulant in these reactions. Dam2 has shown that material which shows the biological activity of vitamin K is contained in chloroplasts and is localized in the green parts of plants. On the other hand, vitamin K1 has not been isolated from chloroplasts and has not been detected previously by chemical assay in extracts from chloroplasts3. In our laboratory we have not previously been able to observe vitamin K1 in extracts from chloroplasts even though we could find the vitamin in extracts from whole leaves. We have now found chemical evidence for vitamin K1 in extracts from spinach chloroplasts. The compound has been purified by silicic acid column chromatography and has shown absorption maxima at 269, 261, 249, 242 mµ in alcoholic solution. After treatment with potassium borohydride the absorption decreases in the 260–270 mµ region and increases to a new maximum at 244 mµ with an isosbestic point at 254 mµ. This material also gives a positive Irrevere–Sullivan test4 as well as a positive Dam–Karar test5 for vitamin K.

References

  1. 1

    Arnon, D. I., Fed. Proc., 20, 1012 (1961).

    CAS  PubMed  Google Scholar 

  2. 2

    Dam, H., Adv. in Enzymology, 2, 285 (1942).

    CAS  Google Scholar 

  3. 3

    Bishop, N. I., in Ciba Symposium on Quinones and Electron Transport,, edit. by Wolstenholme, G. E., and O'Connor, C. M., 385 (Churchill, London, 1961).

    Google Scholar 

  4. 4

    Irrevere, F., and Sullivan, M. X., Science, 94, 497 (1941).

    ADS  Article  Google Scholar 

  5. 5

    Dam, H., Geiger, A., Glavind, J., Karrer, P., Karrer, W., Rothschild, E., and Salomon, H., Helv. Chem. Acta, 22, 310 (1939).

    CAS  Article  Google Scholar 

  6. 6

    Lester, R. L., and Ramasarma, T., J. Biol. Chem. (in the press).

  7. 7

    Dam, H., Glavind, J., and Nielsen, N., Z. Physiol. Chem., 265, 80 (1940).

    CAS  Article  Google Scholar 

  8. 8

    Crane, F. L., Plant Physiol., 34, 128 (1959).

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

KEGEL, L., CRANE, F. Vitamin K1 in Chloroplasts. Nature 194, 1282 (1962). https://doi.org/10.1038/1941282a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing