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B12 Coenzyme Content of the Nodules from Legumes, Alder and of Rhizobium meliloti


IT has been established1 that cobalt is an essential element for the symbiotic growth of soybean plants and Rhizobium japonicum in a purified solution lacking-combined nitrogen. It has been shown also that cobalt is needed for alfalfa inoculated with Rhizobium meliloti2. Legumes supplied with adequate fixed nitrogen do not respond to additions of cobalt1,2; but R. japonicum, R. meliloti, R. leguminosarum, R. trifolii, and R. phaseoli cultured in a purified medium containing both ammonium and nitrate nitrogen failed to grow normally without cobalt3,4. The vitamin B12 content of root nodules from soybean plants is positively correlated with the cobalt supply in the nutrient medium1, suggesting that an important role of this element in nodule metabolism is related to that of the vitamin.

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KLIEWER, M., EVANS, H. B12 Coenzyme Content of the Nodules from Legumes, Alder and of Rhizobium meliloti. Nature 194, 108–109 (1962).

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