ASCORBIC acid has been utilized extensively in large doses in the therapy of industrial methæmoglobinæmia, although Halsted1 commented that at developments greater than 60 per cent methæmoglobinæmia, it is ineffective because of its lack of ability to bring on rapidly a reversion mechanism. But the rapidity of reversion is only the first problem. The second problem to be settled is whether, in chemical cyanosis, the effect of ascorbic acid may not be restricted only to bring about the reduction. It is known that ascorbic acid promotes the rate of hydroxylation of aniline to p-aminophenol2, which is a potent agent in the production of Heinz bodies not only in vivo but also in vitro3, and ascorbic acid is able to produce Heinz bodies in vitro without any toxic agents4. We have found that in rats poisoned with aniline the formation of Heinz bodies is promoted by treatment with ascorbic acid.
Halsted, H. C., J. Occup. Med., 2, 591 (1960).
Axelrod, J., Udenfriend, S., and Brodie, B. B., J. Pharmacol. Exp. Therap., 111, 176 (1954).
Künzer, W., Ambs, E., and Schneider, D., Z. Kinderheilk., 74, 652 (1954).
Gajdos, A., and Tiprez, G., C.R. Soc. Biol., 139, 545 (1945).
Evelyn, K. A., and Malloy, H. T., J. Biol. Chem., 126, 655 (1938).
Magos, L., Indust. Med. Surg., 29, 593 (1960).