Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Separation of Anthocyanins by Cellulose Column Chromatography

Nature volume 193, pages 801–802 (1962)Cite this article

Abstract

PAPER chromatography has greatly facilitated the separation, isolation and identification of anthocyanins and other water-soluble plant pigments1. Large-scale separations have been accomplished by means of adsorption chromatography on cellulose2,3 or magnesol4 columns. Attempts to obtain distinct bands of anthocyanins using cellulose column chromatography according to Endo's3 procedure were unsuccessful. A method used by Chorney5 to separate sugars quantitatively by means of cellulose column chromatography has proved to be eminently satisfactory for large-scale separation of a mixture of anthocyanins.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Harbone, J. B., Chromat. Rev., 1, 209 (1959).

    Article  Google Scholar 

  2. Ice, C. H., Gage, T. B., and Wender, S. H., Proc. Oklahoma Acad. Sci., 32, 99 (1951).

    CAS  Google Scholar 

  3. Endo, T., Nature, 179, 378 (1957).

    Article  ADS  CAS  Google Scholar 

  4. Ice, C. H., and Wender, S. H., Anal. Chem., 24, 1616 (1952).

    Article  CAS  Google Scholar 

  5. Chorney, W., Quart. Rep. Biol. and Med. Res. Div., Argonne Nat. Lab., ANL-5426, 159 (1955).

  6. Garber, E. D., Bot. Gaz., 119, 240 (1958).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, University of Chicago, and Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois

    E. D. GARBER, W. F. REDDING & W. CHORNEY

Authors
  1. E. D. GARBER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. F. REDDING
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. CHORNEY
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

GARBER, E., REDDING, W. & CHORNEY, W. Separation of Anthocyanins by Cellulose Column Chromatography. Nature 193, 801–802 (1962). https://doi.org/10.1038/193801b0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/193801b0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing