Letter | Published:

Formation of γ-Guanidinobutyric Acid in Pine Tissues

Naturevolume 193page781 (1962) | Download Citation



IN several experiments in which arginine uniformly labelled with carbon-14 was fed to various tissues of pines, one unidentified compound was found to contain most of the radioactivity incorporated into alcohol-soluble products. When tissue extracts were cleared for chromatography following the ion-exchange procedure of Plaisted1, the unknown was eluted from ‘Dowex 50’ resin with the acidic and neutral amino-acids. However, the RF of the unidentified compound on paper chromatography did not correspond with the RF of any common amino-acid. Subsequent tests indicated that the radioactive unknown was γ-guanidinobutyric acid, previously found in several species of higher plants, as well as in insects and mammals, by Irreverre et al.2.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Plaisted, P. H., Contrib. Boyce Thompson Inst., 19, 231 (1958).

  2. 2

    Irreverre, F., Evans, R. L., Hayden, A. R., and Silber, R., Nature, 180, 704 (1957).

  3. 3

    Benson, A. A., Bassham, J. A., Calvin, M., Goodale, T. C., Haas, V. A., and Stepka, W., J. Amer. Chem. Soc., 72, 1710 (1950).

  4. 4

    Rivard, D. E., and Carter, H. E., J. Amer. Chem. Soc., 77, 1260 (1955).

  5. 5

    Thoai, N., Roche, J., and Robin Yvonne, C.R. Acad. Sci., Paris, 235, 832 (1952).

  6. 6

    Pisano, J. J., Mitoma, C., and Udenfriend, S., Nature, 180, 1125 (1957).

Download references

Author information


  1. Southeastern Forest Experiment Station, Forest Service, U.S. Department of Agriculture, Duke University, Durham, North Carolina



  1. Search for ROBERT L. BARNES in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.