Letter | Published:

Purification of a New b-Type Cytochrome from Mung Bean (Phaseolus aureus) Seedlings

Naturevolume 193pages776777 (1962) | Download Citation



ON the basis of spectral observations, four b-type cytochromes (b, b3, b6, b7) have been identified in higher plant tissues1. Of these pigments, all of which are bound firmly to the cytoplasmic particles, only cytochrome b3 has been extensively purified2. In the course of an examination of the cytochromes in etiolated mung bean seedlings, we have isolated a soluble pigment which has the properties of a b-type cytochrome; it has an unusual α-band (reduced), with a peak at 555 and a shoulder at 559 mµ. The purification and some of the properties of this component are described here.

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  1. 1

    Hartree, E. F., Adv. in Enzymol., 18, 1 (1957).

  2. 2

    Hill, R., and Scarisbrick, R., New Phytol., 50, 98 (1951).

  3. 3

    Hartree, E. F., Modern Methods of Plant Analysis, edit. by Paech, K., and Tracey, M. V., 4, 197 (Springer-Verlag, Berlin, 1955).

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  1. Department of Biochemistry, University of California, Berkeley

    • H. SHICHI
    •  & D. P. HACKETT


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