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Simultaneous Staining of Heinz Bodies and Reticulocytes with New Methylene Blue N in Uromastix acanthinurus after Iproniazid Treatment

Naturevolume 193page594 (1962) | Download Citation



IN dogs given iproniazid (‘Marsilid’) it was observed that Brecher's method1 for counting reticulocytes very clearly showed the presence of Heinz bodies2, which yielded a method for demonstration of both Heinz bodies and reticulocytes in the same preparation3. Since, to the best of my knowledge, nothing is known of the possible occurrence of Heinz bodies and reticulocytes in lower vertebrates, I performed a corresponding investigation with the lizard Uromastix acanthinurus. As staining technique the method of Brecher1 was used according to Thompson's method3. The erythrocytes appeared to be stained a pale greenish-blue, while the reticulum was deep blue and sharply outlined. The Heinz bodies stood out prominently against the pale-green background of the erythrocytes and were pale to deep blue. As in the dog erythrocytes3, these bodies were stained at least as well as by the usual methods2,4,5. As these results correspond very well with those obtained in the dog3 a more general importance should no doubt be attached to it.

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  1. 1

    Brecher, G., Amer. J. Clin. Path., 19, 895 (1949).

  2. 2

    Heinz, R., Berl. klin. Wochenschr., 27, 47 (1890).

  3. 3

    Thompson, E. C., Stain Tech., 36, 38 (1961).

  4. 4

    Webster, S. H., Liljegren, E. J., and Zimmer, D. J., Stain Tech., 23, 97 (1948); J. Pharmacol. Exp. Therap., 95, 201 (1949).

  5. 5

    Spicer, S. S., and Thompson, E. C., J. Indust. Hyg. Tox., 31, 206 (1949).

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  1. Department of Histology, Free University, Amsterdam

    • A. STOLK


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