Abstract
FOR the past several years, Ehrlich ascites tumour cells have been used in this Laboratory in work on the anti-tumour activity of fatty acids1–3. Serial transplantation of the stock tumour in Swiss mice was carried out at weekly intervals using an inoculum of 15 × 106 washed ascites cells, a number which effected a maximum rate of cell proliferation4. Under these conditions, the proportion of tumour cells in the ascitic fluid reached 60–70 per cent within 5–6 days after inoculation. Afterwards, however, the percentage of tumour cells declined steadily and dropped to only 10–20 per cent shortly before death of the mice at 10–14 days. This observation suggested that the ascitic fluid might be exerting an oncolytic effect on the tumour cells5,6, and experiments were carried out to test the activity of the fluid after removal of the tumour cells by high-speed centrifugation. These experiments, rather than revealing an oncolytic factor, indicated the presence of a transmissible agent producing ascites tumours.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Townsend, G. F., Morgan, J. F., and Hazlett, B., Nature, 183, 1270 (1959).
Townsend, G. F., Morgan, J. F., Tolnai, S., Hazlett, B., Morton, H. J., and Shuel, R. W., Cancer Res., 20, 503 (1960).
Morgan, J. F., Tolnai, S., and Townsend, G. F., Canad. J. Biochem. and Physiol., 38, 597 (1960).
Klein, G., and Révész, L., J. Nat. Cancer Inst., 14, 229 (1953).
Bennette, J. G., Nature, 187, 72 (1960).
Nelson, J. B., and Tarnowski, G. S., Nature, 188, 866 (1960).
Hanks, J. H., and Wallace, R. E., Proc. Soc. Exp. Biol. Med., 71, 196 (1949).
Klein, G., Cancer Res., 12, 589 (1952).
Friend, C., J. Exp. Med., 105, 307 (1957).
Selby, C. C., Biesele, J. J., and Grey, C. E., Ann. N.Y. Acad. Sci., 63, 748 (1956).
Schmidt, F., Z. F.D. Gesamte Innere Med., 11, 640 (1956).
Graffi, A., Bielka, H., and Fey, F., Acta Hæmat., 15, 145 (1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TOLNAI, S., SCANTLAND, R. & MORGAN, J. Sub-cellular Transmissible Agent from Ehrlich Carcinoma Cells producing Ascites Tumours in Mice. Nature 193, 591–592 (1962). https://doi.org/10.1038/193591b0
Issue Date:
DOI: https://doi.org/10.1038/193591b0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.