Letter | Published:

Extinction Coefficient at Zero Concentration

Nature volume 193, pages 564565 (10 February 1962) | Download Citation

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Abstract

THE use of extinction coefficients to characterize substances depends on the validity of Lambert–Beer's law. The following shows that a constant-extinction coefficient at zero concentration—which is the tangent to the extinction–concentration curve at zero concentration, may be calculated even if Lambert–Beer's law is not followed.

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Affiliations

  1. Institute for Medical Biochemistry and Physiology, University of Oslo.

    • J. FOG

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DOI

https://doi.org/10.1038/193564a0

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