Letter | Published:

Identification of the Main Ethylenediamine Condensate of Noradrenaline with that of Catechol

Naturevolume 193pages484485 (1962) | Download Citation



BECAUSE of the importance of fluorometric microanalysis of catecholamines, the ethylenediamine condensation method1 should be examined in detail. The specificity of this method for noradrenaline might be important. In previous communications2,3, we indicated that the main condensation product of noradrenaline or of 3,4-dihydroxymandelic acid is considered to be the same as that of catechol, and that the side-chain of noradrenaline or of 3,4-dihydroxymandelic acid might be damaged severely during the condensation reaction. Weil-Malherbe4 reported that with catechol, noradrenaline and 3,4-dihydroxymandelic acid, two main products which appear to be identical for the three compounds are formed. According to Joly and Nadeau5, at least three of the fluorescent spots on a two-dimensional paper chromatogram of an ethylenediamine condensate of tagged adrenaline (β-14C-DL-adrenaline) were not radioactive, and these three fluorescent spots were found to be common to the three chromatograms obtained from adrenaline, noradrenaline and catechol.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Weil-Malherbe, H., and Bone, A. D., Biochem. J., 51, 311 (1952).

  2. 2

    Yagi, K., and Nagatsu, T., Nature, 183, 822 (1959).

  3. 3

    Yagi, K., Nagatsu, T., and Nagatsu, I., Nature, 186, 310 (1960).

  4. 4

    Weil-Malherbe, H., Biochim. Biophys. Acta, 40, 351 (1960).

  5. 5

    Joly, L. P., and Nadeau, G., Nature, 184, 1438 (1959).

  6. 6

    Nadeau, G., and Joly, L. P., Nature, 182, 180 (1958).

  7. 7

    Gray, I., and Young, J. G., Clin. Chem., 3, 239 (1957).

Download references

Author information


  1. Department of Biochemistry, School of Medicine, Nagoya University, Nagoya

    •  & KUNIO YAGI


  1. Search for TOSHIHARU NAGATSU in:

  2. Search for KUNIO YAGI in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.