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Transformation to Prototrophic Condition in Escherichia coli induced by Deoxyribonucleic Acid

Nature volume 193, pages 296–297 (1962)Cite this article

Abstract

SINCE the demonstration by Avery et al.1 of type transformation induced in Pneumococcus by deoxyribonucleic acid (DNA) several markers have been used to study the phenomenon in bacteria including drug resistance2, protein specificity3, and colony morphology4. Chargaff et al.5 have demonstrated “redintegration”, a process of rehabilitation in the protoplasts of a lysine-requiring mutant of Escherichia coli. In the present work it has been demonstrated that a biologically active DNA preparation can be readily obtained from the cells of Escherichia coli and that intact cells can act as recipients of this material and undergo transformation.

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References

  1. Avery, O. T., McLeod, C. M., and McCarty, M., J. Exp. Med., 79, 137 (1944).

    Article  CAS  Google Scholar 

  2. Hotchkiss, R. D., Cold Spring Harbor Symposia Quant. Biol., 16, 457 (1951).

    Article  CAS  Google Scholar 

  3. Austrian, R., and McLeod, C. M., J. Exp. Med., 89, 451 (1949).

    Article  CAS  Google Scholar 

  4. Ephrussi Taylor, H., Cold Spring Harbor Symposia Quant. Biol., 16, 445 (1951).

    Article  CAS  Google Scholar 

  5. Chargaff, E., Schulmann, H. M., and Shapiro, H. S., Nature, 180, 851 (1957).

    Article  ADS  CAS  Google Scholar 

  6. Davis, B. D., and Mingioli, E. S., J. Bact., 60, 17 (1950).

    CAS  PubMed  Google Scholar 

  7. McCarty, M., and Avery, O. T., J. Exp. Med., 83, 97 (1946).

    Article  CAS  Google Scholar 

  8. Ross, G. I. M., J. Clin. Path., 5, 250 (1952).

    Article  CAS  Google Scholar 

  9. Green, M. N., and Sevag, M. G., Arch. Biochem., 9, 129 (1946).

    CAS  PubMed  Google Scholar 

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Author notes

  1. A. SREENIVASAN

    Present address: Deputy Director, Central Food Technological Research Institute, Mysore

Authors and Affiliations

  1. Department of Chemical Technology, University of Bombay, Bombay, 19

    B. M. MEHTA, D. V. REGE & A. SREENIVASAN

Authors
  1. B. M. MEHTA
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  2. D. V. REGE
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  3. A. SREENIVASAN
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MEHTA, B., REGE, D. & SREENIVASAN, A. Transformation to Prototrophic Condition in Escherichia coli induced by Deoxyribonucleic Acid. Nature 193, 296–297 (1962). https://doi.org/10.1038/193296a0

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  • DOI: https://doi.org/10.1038/193296a0

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