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Oxidized Cytochrome and Chlorophyll C2 + in Photosynthesis

Naturevolume 192pages967969 (1961) | Download Citation



CHANCE et al.1a reported the presence of trapped oxidized cytochrome in bacteria at −200° C., and in a previous communication1b we supplied evidence for the presence of trapped oxidized cytochrome during photosynthesis of green plants at −150° C. We have now refined and extended the measurements over the whole range of the visible spectrum. The difference spectrum of the trapped products in fresh spinach chloroplasts is shown in Fig. la. It shows in addition to the oxidized cytochrome Cyt + (solid line) the trapping of what is probably oxidized chlorophyll C + (dotted line). Both substances are products of one and the same light reaction hv1. It has been shown that in green plants photosynthesis is triggered by two light reactions hv1 and hv2, one being sensitized by a chlorophyll pigment already sensitive at 710 mµ and the other sensitized by a chlorophyll pigment (P) sensitive at shorter wave-lengths (see refs. 2 and 3). Exciting only hv1 (at + 20° C. and 710 mµ) gives almost the same difference spectrum as presented in Fig. 1a. Two light-reactions in photosynthesis are discussed in the literature2,5,6,8.

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  1. 1

    a Chance, B., and Nishimura, M., Proc. U.S. Nat. Acad. Sci., 46, 12 (1960). b Müller, A., and Witt, H. T., Nature, 189, 944 (1961).

  2. 2

    Witt, H. T., Müller, A., and Rumberg, B., Nature, 191, 194 (1961).

  3. 3

    Witt, H. T., Müller, A., and Rumberg, B., Angew. Chem., 73, 507 (1961).

  4. 4

    Vernon, L. P., and Kamen, M. D., Arch. Biochem. Biophys., 51, 122 (1954). Vernon, L. P., and Zaugg, W. S., Biochem., 235, 2728 (1960).

  5. 5

    Kok, B., Acta Botan. Neerl., 6, 316 (1957). Kok, B., and Hoch, G., Light and Life, 397, ed. by McElroy and Glass (Johns Hopkins Press, 1961).

  6. 6

    Losada, M., Whatley, F. R., and Arnon, D. J., Nature, 190, 606 (1961).

  7. 7

    Kok, B., Biochim. Biophys. Acta, 48, 527 (1961).

  8. 8

    Allen, M. B., Pietti, L. H., and Murchio, J. C., Biochem. Biophys. Res. Comm., 4, 271 (1961). Duysens, L. N. M., Amesz, J., and Kamp, B. M., Nature, 190, 510 (1961).

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  1. Physikalisch-Chemisches Institut, Universität Marburg/Lahn, Germany

    • H. T. WITT
    • , A. MÜLLER
    •  & B. RUMBERG


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