Letter | Published:

Quantitative Estimation of Peak Areas in Gas-Liquid Chromatography

Naturevolume 192page965 (1961) | Download Citation



RECENTLY, Carroll1 advocated the use of the product of retention time (tR) and peak height (h) for the quantitative estimation of relative peak areas. This method2, based on the assumption of a proportionality of peak width (w) and retention time (tR) or volume (VR), gives satisfactory results with respect to the reproducibility. In fact, we have found that the reproducibility (under equal operating conditions) of this method is better than that of any other ‘manual’ estimation of peak areas. However, the method is not commonly used in our Laboratory, as the results obtained with it are erratic. This is due to the fact that in most cases the proportionality between w and VR is only approximate. Differences between the relative areas, as found by the triangulation method and from the product of h and VR, can amount to several per cent relative. In principle the non-linearity of w and VR can be taken into account when calibrating the detector. However, changing the column or altering the carrier gas velocity will change the calibration factors.

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  1. 1

    Carroll, K. K., Nature, 191, 377 (1961).

  2. 2

    Phillips, C., Gas Chromatography, 63 (Butterworth, London, 1956).

  3. 3

    Keulemans, A. I. M., Gas Chromatography, 127 (Reinhold Pub., New York, 1959).

  4. 4

    van Deemter, J. J., Zuiderweg, F. J., and Klinkenberg, A., Chem. Eng. Sci., 5, 271 (1956).

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  1. Central Laboratory, Staatsmijnen in Limburg, Geleen, The Netherlands



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