Abstract
THE effect of paramagnetic ions on proton relaxation times depends not only on their concentration but also on the binding of the ion to other molecules which may be present in the same solution. By measuring longitudinal relaxation times (T1) by nuclear magnetic resonance we have compared the proton relaxation-rates at constant ion concentration with and without deoxyribonucleic acid (DNA) present. In this way it was possible to detect binding and to distinguish between interior and exterior DNA binding sites for different transition metal ions.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Carr, H. Y., and Purcell, E. M., Phys. Rev., 88, 415 (1952).
Solomon, I., Phys. Rev., 99, 559 (1955).
Bloembergen, N., J. Chem. Phys., 27, 572, 595 (1957).
See Davidson, N., and Gold, R., Biochim. Biophys. Acta, 26, 370 (1957).
Felsenfeld, G., and Huang, S., Biochim. Biophys. Acta, 51, 19 (1961).
Brooks, P., quoted in Yamane, T., and Davidson, N., J. Amer. Chem. Soc., 83, 2599 (1961).
Fuwa, K., Wacker, W. E. C., Druyan, R., Bartholomay, A. F., and Vallee, B. L., Proc. U.S. Nat. Acad. Sci., 46, 1298 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
EISINGER, J., SHULMAN, R. & BLUMBERG, W. Relaxation Enhancement by Paramagnetic Ion Binding in Deoxyribonucleic Acid Solutions. Nature 192, 963–964 (1961). https://doi.org/10.1038/192963a0
Issue Date:
DOI: https://doi.org/10.1038/192963a0
This article is cited by
-
Magnetic resonance imaging of brain cell water
Scientific Reports (2019)
-
Magnetic resonance imaging of noradrenergic neurons
Brain Structure and Function (2019)
-
Reduced intracellular mobility underlies manganese relaxivity in mouse brain in vivo: MRI at 2.35 and 9.4 T
Brain Structure and Function (2015)
-
Approximation of weak stability constants of paramagnetic complexes by spin-lattice relaxation measurements of water protons
Applied Magnetic Resonance (1994)
-
The lanthanides as spectroscopic and magnetic resonance probes in biological systems
Die Naturwissenschaften (1975)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.