Letter | Published:

Binding of RP2-L—a Nuclear Protein of Neoplastic Tissues—to Deoxyribonucleic Acid

Nature volume 192, pages 870871 (02 December 1961) | Download Citation

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Abstract

RECENT work in this laboratory has indicated that the incorporation of radioactive amino-acids into nuclear proteins, especially the acid-soluble nuclear proteins, represents an important metabolic pathway in transplantable rat tumours1–3. The acid-soluble nuclear proteins obtained from rats injected with L-lysine-U-14C were chromatographed on carboxymethyl-cellulose columns4 after the acid extracts had been dialysed against 0.5 N acetic acid. The cellulose columns (sodium form) had been equilibrated against 0.05 M sodium acetate buffer, pH 4.0. Gradient elution was used with N formic acid followed by 8 N formic acid. All procedures were carried out at 4° C. The resolving power of this method provided specific chromatographic patterns for protein distribution and distribution of radioactivity for the acid-soluble nuclear proteins of the Walker tumour and various tissues of the tumour-bearing rat. The chromatograms of the acid-soluble nuclear proteins from the various non-tumour tissues contained one to three radioactive peaks with relatively low specific activities. The chromatograms of the nuclear extracts of the Walker tumour, and a number of other malignant tumours, contained a radioactive peak which was not found in any of the growing or non-growing4,5 non-tumour tissues studied.

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References

  1. 1.

    , , and , Cancer Research, 18, 926 (1958).

  2. 2.

    , , , , , and , Cancer Research, 19, 1030 (1959).

  3. 3.

    , and , Cancer Research, 18, 718 (1958).

  4. 4.

    , and , Cancer Research, 19, 1157 (1959).

  5. 5.

    , and , Cancer Research, 20, 1208 (1960).

  6. 6.

    , , and , Cancer Research, 19, 684 (1959).

  7. 7.

    , Arkiv Kemi Mineral. Geol., 24A, 31 (1947).

  8. 8.

    , Bull. Soc. Chim. Biol., 36, 15 (1954).

  9. 9.

    , , and , J. Biol. Chem., 206, 499 (1954).

  10. 10.

    , , and , Arch. Biochim. Biophys., 58, 144 (1955).

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Affiliations

  1. Department of Pharmacology and Biochemistry, Baylor University College of Medicine, Houston 25, Texas.

    • PAUL BYVOET
    •  & HARRIS BUSCH

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DOI

https://doi.org/10.1038/192870a0

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