Abstract
IT has been shown that the histochemical staining reaction for tissue glycogen depends on the amount of lyoglycogen present in the tissues1. The present communication is a preliminary report of the nature of lyoglycogen. Lyoglycogen was obtained from the liver of six guinea pigs, two of which had been starved for 72 hr. and others that had had a normal diet. Hydrolysis of the resultant samples of lyoglycogen was carried out by refluxing with N/l sulphuric acid at 100° C. for 8 hr. The acid solution was neutralized with barium carbonate, filtered and concentrated in vacuo. The solutions were applied to Whatman 3 MM papers which were developed in n-propanol–ethylacetate–water for 15–18 hr. Locating-reagents used were aniline-diphenylamine and silver nitrate.
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References
Kugler, J. H., and Wilkinson, W. J. C., J. Histochem. Cytochem., 8, 195 (1960).
Rosenfeld, E. L., Biokhimiya, 23, 877 (1958).
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WILKINSON, W., KUGLER, J. Variation in Lyoglycogen in Starved and Normal Guinea Pigs. Nature 192, 466 (1961). https://doi.org/10.1038/192466a0
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DOI: https://doi.org/10.1038/192466a0
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