Abstract
IT has been shown previously1 that rat liver nuclei isolated in 0.25 M sucrose solution will incorporate 14C-glycine into protein in vitro. The possibility of non-specific exchange of glycine was eliminated2. It was shown that radioactivity was retained after re-incubation of labelled nuclei for 2 hr. in a 200-fold excess of unlabelled glycine. The incorporation of glycine by nuclear preparations was inhibited by 2,4-dinitrophenol (DNP), azide, and anoxia.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Rees, K. R., and Rowland, G. F., Biochem. J., 78, 89 (1961).
Allfrey, V. G., Mirsky, A. E., and Osawa, S., J. Gen. Physiol., 40, 451 (1957).
Frenster, J. H., Allfrey, V. G., and Mirsky, A. E., Proc. U.S. Nat. Acad. Sci., 46, 432 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
REES, K., ROSS, H. & ROWLAND, G. Incorporation of 14C-glycine by Sub-Nuclear Preparations from Rat Liver Nuclei. Nature 192, 171–172 (1961). https://doi.org/10.1038/192171a0
Issue Date:
DOI: https://doi.org/10.1038/192171a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.