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Chromosome Preparation from Fresh and Cultured Tissues using a Modification of the Drying Technique

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Abstract

TECHNIQUES for making satisfactory chromosome preparations have evolved rapidly during the past decade since Makino and Nishimura1 and Sachs2 adopted pre-treatment with hypotonic solutions and the squash method of preparation. Administration of colchicine increased the number of countable mitoses and facilitated analysis by arresting cell division in metaphase, producing shortening of chromosomes and divergence of sister chromatids.

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References

  1. 1

    Makino, S., and Nishimura, I., Stain Tech., 27, 1 (1952).

  2. 2

    Sachs, L., Heredity, 6, 357 (1952).

  3. 3

    Darlington, C. D., and La Cour, L. F., The Handling of Chromosomes, second ed. (George Allen and Unwin, Ltd., London).

  4. 4

    Tough, I. M., Court Brown, W. M., Baikie, A. G., Buckton, K. E., Harnden, D. G., Jacobs, P. A., King, M. J., and McBride, J. A., Lancet, i, 411 (1961).

  5. 5

    Rothfels, K. H., and Siminovitch, L., Stain Tech., 33, 73 (1958).

  6. 6

    Moorhead, P. S., Nowell, P. C., Mellman, W. J., Battips, D. M., and Hungerford, D. A., Exp. Cell Res., 20, 613 (1960).

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https://doi.org/10.1038/1921213a0

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