Abstract
PREVIOUS work has revealed a specific requirement of a number of marine bacteria for sodium for growth1,2 and oxidative metabolism3,4. For optimum rate and extent of growth, 0.2–0.3 M Na+ was required though limited growth could occur after a sufficiently long incubation period at as low as 0.03 M Na+ (ref. 2). Since sucrose and K+ exerted only a slight sparing action on the Na+ requirement of the organisms examined it was concluded that the maintenance of an appropriate osmotic pressure in the medium was by no means the sole, or even the primary, function of Na+ in the growth of the cells. Nevertheless, cells of many of the species lysed quickly when washed in distilled water, and in the case of one of the species examined in more detail, lysis occurred also when cells were suspended in solutions containing sodium chloride and potassium chloride at concentrations of these salts that were optimum for growth. Lysis, however, did not occur when 0.05 Mg2+ or Mn2+ was included in the suspending medium3.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
MacLeod, R. A., Onofrey, E., and Norris, M. E., J. Bact., 68, 680 (1954).
MacLeod, R. A., and Onofrey, E., J. Cell and Comp. Physiol., 50, 389 (1957).
Tomlinson, N., and MacLeod, R. A., Canad. J. Microbiol., 3, 627 (1957).
MacLeod, R. A., Claridge, C. A., Hori, A., and Murray, J. F., J. Biol. Chem., 232, 829 (1958).
MacLeod, R. A., and Onofrey, E., Canad. J. Microbiol., 3, 753 (1957).
Mager, J., Kuczynski, M., Schatzberg, G., and Avi-Dor, Y., J. Gen Microbiol., 14, 69 (1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MACLEOD, R., MATULA, T. Solute Requirements for preventing Lysis of Some Marine Bacteria. Nature 192, 1209–1210 (1961). https://doi.org/10.1038/1921209b0
Issue Date:
DOI: https://doi.org/10.1038/1921209b0
This article is cited by
-
A preliminary investigation on the growth requirement for monovalent cations, divalent cations and medium ionic strength of marine actinomycete Salinispora
Applied Microbiology and Biotechnology (2010)
-
Stabilization of Autolysis in Bacillus cereus T
Nature (1966)
-
A Role for Inorganic Ions in the Maintenance of Intracellular Solute Concentrations in a Marine Pseudomonad
Nature (1965)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.