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Purification and Concentration of Dye-Protein Conjugates by Gel Filtration

Naturevolume 192pages11881189 (1961) | Download Citation



IT was shown by Porath and Flodin1 that filtration through cross-linked dextran gels served as a simple method for the separation of materials of varying molecular size. This procedure has now been found to be of particular value for the purification of antibody-fluorescent dye conjugates for use in the Coon's2 technique, since unconjugated dye and dye impurities are removed so that non-specific staining of the tissues has thereby been greatly reduced or abolished.

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  1. 1

    Porath, J., and Flodin, P., Nature, 183, 1657 (1959).

  2. 2

    Coons, A. H., Creech, H. J., Jones, R. N., and Berliner, E., J. Immunol., 45, 159 (1942).

  3. 3

    Riggs, J. L., Seiward, J. R., Burckhalter, J. H., and Downs, C. M., Amer. J. Path., 34, 1081 (1958).

  4. 4

    Marshall, J. D., Eveland, W. C., and Smith, C. W., Proc. Soc. Exp. Biol. (N.Y.), 98, 898 (1958).

  5. 5

    Lever, W. F., Gurd, F. R. N., Uroma, E., Brown, R. K., Barnes, B. A., Schmid, K., and Schultz, E., J. Clin. Invest., 30, 99 (1951).

  6. 6

    Ouchterlony, O., Acta Path. Microbiol. Scand., 32, 231 (1953).

  7. 7

    Gell, P. G. H., J. Clin. Path., 10, 67 (1957).

  8. 8

    Flodin, P., Gelotte, B., and Porath, J., Nature, 188, 493 (1960).

  9. 9

    Creech, H. J., and Jones, R. N., J. Amer. Chem. Soc., 63, 1661 (1941); 63, 1670 (1941). Dineen, J. K., and Ada, G. L., Nature, 180, 1284 (1957).

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  1. Department of Experimental Pathology, University of Birmingham

    • W. GEORGE
    •  & K. W. WALTON


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