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Purification and Concentration of Dye-Protein Conjugates by Gel Filtration

Nature volume 192, pages 1188–1189 (1961)Cite this article

Abstract

IT was shown by Porath and Flodin1 that filtration through cross-linked dextran gels served as a simple method for the separation of materials of varying molecular size. This procedure has now been found to be of particular value for the purification of antibody-fluorescent dye conjugates for use in the Coon's2 technique, since unconjugated dye and dye impurities are removed so that non-specific staining of the tissues has thereby been greatly reduced or abolished.

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Authors and Affiliations

  1. Department of Experimental Pathology, University of Birmingham,

    W. GEORGE & K. W. WALTON

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  1. W. GEORGE
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  2. K. W. WALTON
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GEORGE, W., WALTON, K. Purification and Concentration of Dye-Protein Conjugates by Gel Filtration. Nature 192, 1188–1189 (1961). https://doi.org/10.1038/1921188a0

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