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Purification and Concentration of Dye-Protein Conjugates by Gel Filtration

Naturevolume 192pages11881189 (1961) | Download Citation

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Abstract

IT was shown by Porath and Flodin1 that filtration through cross-linked dextran gels served as a simple method for the separation of materials of varying molecular size. This procedure has now been found to be of particular value for the purification of antibody-fluorescent dye conjugates for use in the Coon's2 technique, since unconjugated dye and dye impurities are removed so that non-specific staining of the tissues has thereby been greatly reduced or abolished.

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References

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    Porath, J., and Flodin, P., Nature, 183, 1657 (1959).

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Affiliations

  1. Department of Experimental Pathology, University of Birmingham

    • W. GEORGE
    •  & K. W. WALTON

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https://doi.org/10.1038/1921188a0

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