Letter | Published:

Investigation with the Electron Microscope of the Sublimation of Ice Crystals at Low Temperatures

Naturevolume 192pages11771178 (1961) | Download Citation



INVESTIGATIONS of ice crystals by means of the electron microscope have been made by several investigators1–4, but most of them were based on replication techniques. Recently, Fernández-Morán5 reported the results obtained by low-temperature microscopy; he succeeded in stabilizing ice crystals deposited on a cold substrate by maintaining them at low temperatures during observation in the electron microscope with a microbeam of very low intensity. It was noted by him that the ice crystals were predominantly cubic and hexagonal in shape at −120° C. and at −100° to 90° C, respectively, and actually showed corresponding sharp-ring diffraction patterns.

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    Hall, C. E., J. App. Phys., 21, 61 (1950).

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    Meryman, H. T., and Kafig, E., Naval Med. Res. Inst. Proj. Rep., N M000 018.01.09, 13, 529 (1955).

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    Honjo, G., Kitamura, N., Shimaoka, K., and Mihama, K., J. Phys. Soc. Japan, 11, 527 (1956).

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    Hibi, T., and Yada, K., J. Electronmicroscopy, Chiba, 7, 21 (1959).

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    Fernández-Morán, H., Ann. New York Acad. Sci., 85, 689 (1960).

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    Nei, T., Biochim. Biophys. Acta, 49, 426 (1961).

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  1. Institute of Low Temperature Science, Hokkaido University, Sapporo



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