Letter | Published:

Gas Chromatography of Androst-16-en-3α-ol and Related Steroids

Naturevolume 192pages10741075 (1961) | Download Citation



DURING the course of work on the in vitro biosynthesis of androst-16-en-3α-ol (androstenol, I) from 2-14C-acetate or 3H-acetate by rabbit and guinea pig testes1, it was found that the highly radioactive saponifiable lipids present could be only partially separated from the slightly active I on alumina, using as eluant light petroleum/benzene (1 : 1, v/v). Although in later experiments the neutral lipids were removed by saponification before column chromatography, it was considered that the technique of gas chromatography might provide an alternative method of separating (I) in view of recent results obtained in the steroid field2–5.

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    Gower, D. B., and Haslewood, G. A. D., J. Endocrinol. (in the press).

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    Sweeley, C. C., and Horning, E. C., Nature, 187, 144 (1960).

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    Brooksbank, B. W. L., Haslewood, G. A. D., Pollock, J. A., and Hewett, C. L., Biochem. J., 70, 14P (1958).

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    VandenHeuvel, W. J. A., Sweeley, C. C., and Horning, E. C., Biochem. Biophys. Res. Comm., 3, 33 (1960).

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    Baker, R. W. R., Biochem. J., 79, 642 (1961).

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    Golay, M. J. E., Gas Chromatography, edit. by Coates, V. J., Noebels, H. J., and Fagerson, I. S., 1 (Academic Press, New York, 1957).

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  1. Departments of Chemical Pathology and Biochemistry, Guy's Hospital Medical School, London, S.E.1

    • R. W. R. BAKER
    •  & D. B. GOWER


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