Letter | Published:

Gas Chromatography of Androst-16-en-3α-ol and Related Steroids

Nature volume 192, pages 10741075 (16 December 1961) | Download Citation

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Abstract

DURING the course of work on the in vitro biosynthesis of androst-16-en-3α-ol (androstenol, I) from 2-14C-acetate or 3H-acetate by rabbit and guinea pig testes1, it was found that the highly radioactive saponifiable lipids present could be only partially separated from the slightly active I on alumina, using as eluant light petroleum/benzene (1 : 1, v/v). Although in later experiments the neutral lipids were removed by saponification before column chromatography, it was considered that the technique of gas chromatography might provide an alternative method of separating (I) in view of recent results obtained in the steroid field2–5.

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Affiliations

  1. Departments of Chemical Pathology and Biochemistry, Guy's Hospital Medical School, London, S.E.1.

    • R. W. R. BAKER
    •  & D. B. GOWER

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DOI

https://doi.org/10.1038/1921074a0

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