A Method of replicating Dry or Moist Surfaces for Examination by Light Microscopy


THE use of transparent replicas is a convenient method for the microscopic study of surfaces. Perfectly dry structures can be replicated with plastic substances such as ‘Collodion’, ‘Formvar’ or methacrylate, but a serious disadvantage to the general use of these materials is that they ‘mist’ when allowed to harden in a moist atmosphere. Accordingly, they cannot be used satisfactorily with specimens that either become distorted when dry or, like many biological specimens, have a naturally wet surface. The following method, which overcomes this difficulty, consists essentially of taking a primary impression of the surface with a silicone rubber plastic and using this to form secondary transparent replicas.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

SAMPSON, J. A Method of replicating Dry or Moist Surfaces for Examination by Light Microscopy. Nature 191, 932–933 (1961). https://doi.org/10.1038/191932a0

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.