Effects of Drugs on Uptake of Isotopic Norepinephrine by Cat Tissues

Abstract

PREVIOUS experiments have shown that catecholamines enter tissue slices both by simple diffusion and by a concentrating mechanism that can be demonstrated in brain, heart and spleen, but not in muscle or liver, when these tissues are incubated with physiological concentrations of isotopic epinephrine or norepinephrine1,2. The uptake by the concentrating mechanism is maximal in media containing 10–25 mµgm. of norepinephrine per ml. and is inhibited by reserpine and ouabain. At concentrations of norepinephrine greater than 200 mµgm. per ml., uptake by the concentrating mechanism becomes negligible in comparison to the amount entering by diffusion and the effect of reserpine is no longer demonstrable.

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References

  1. 1

    Titus, E., Wilson, C. W. M., and Dengler, H. J., Fed. Proc., 19, 297 (1960).

  2. 2

    Brodie, B. B., Dengler, H. J., Titus, E., and Wilson, C. W. M., J. Physiol. (in the press).

  3. 3

    Dengler, H., Arch. Exp. Path. Pharmakol., 231, 373 (1957).

  4. 4

    Burn, J. H., and Rand, M. J., J. Physiol., 144, 314 (1958).

  5. 5

    Holzbauer, M., and Vogt, M., J. Neurochem., 1, 8 (1956).

  6. 6

    Carlsson, A., Rosengren, E., Bertler, A., and Nilsson, J., in Psychotropic Drugs, edit. by Garattini, S., and Ghetti, V., 363 (Elsevier Pub. Co., Amsterdam and New York, 1957).

  7. 7

    Brodie, B. B., Olin, J. S., Kuntzman, R., and Shore, P. A., Science, 125, 1293 (1957).

  8. 8

    Cass, R., Kuntzman, R., and Brodie, B. B., Proc. Soc. Exp. Biol. Med., 103, 870 (1960).

  9. 9

    Hughes, F. B., and Brodie, B. B., J. Pharmacol. Exp. Therap., 127, 96 (1959).

  10. 10

    Fleckenstein, A., and Bass, H., Arch. Exp. Path. Pharmakol., 220, 143 (1953).

  11. 11

    Bein, H. J., Experientia, 9, 107 (1953).

  12. 12

    Boura, A. L. A., and Green, A. F., Brit. J. Pharmacol., 14, 536 (1959).

  13. 13

    Martin, W. R., Riehl, J. L., and Unna, K. R., J. Pharmacol. Exp. Therap., 130, 37 (1960).

  14. 14

    Koelle, G. B., Pharmacol. Rev., 11, 381 (1959).

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DENGLER, H., SPIEGEL, H. & TITUS, E. Effects of Drugs on Uptake of Isotopic Norepinephrine by Cat Tissues. Nature 191, 816–817 (1961). https://doi.org/10.1038/191816a0

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