Letter | Published:

Estimation of Protein in Cellular Material

Naturevolume 191pages492493 (1961) | Download Citation



PROTEIN content is a widely used reference value for microbial growth yield, enzyme activity, and other biochemical quantities. The assay of dissolved protein is relatively easy, being accomplished by measuring the absorbence at 280 mµ or by use of the biuret reagent, etc. The assay of protein in cellular material involves a time-consuming digestion (Kjeldahl method). In addition to being slow, this method is non-specific and does not immediately distinguish between proteins and other nitrogen compounds.

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    Cornell, A., Bardawill, C., and David, M., J. Biol. Chem., 177, 751 (1949).

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  1. Department of Agricultural Biochemistry, University of Vermont, Burlington

    •  & D. B. JOHNSTONE


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