Letter | Published:

Non-Specific Immunity in Pneumococcal Infection of Mice

Naturevolume 191pages295296 (1961) | Download Citation

Subjects

Abstract

THE development of early, non-specific protection against experimental infections with Gram-negative micro-organisms has been observed following injection of bacterial endotoxin, lipopolysaccharide and lipid A1. Reports of similar activities of these substances against infections with Gram-positive organisms are less numerous and have been substantiated only in the case of infection with Staphylococcus pyogenes or Mycobacterium fortuitum2. In an attempt to assess the role of phagocytosis by cells of the liver and spleen in this non-specific protection, we have utilized certain fatty acid esters which are capable of profoundly increasing or reducing the activity of these cells3. One of the experimental models selected for this work has been infection with Streptococcus pneumoniae in mice. Infections with this organism have usually been carried out by the intraperitoneal route and are highly lethal, unless the infection is modified by the classical means of active or passive immunization. In an attempt to demonstrate other, perhaps less potent, protective mechanisms, we felt that a less-virulent type of infection might be more appropriate. This has been achieved by using a strain of Strep. pneumoniae type 8, preparing the challenge inoculum in 0.1 M phosphate buffer at pH 7.4 and injecting the chosen dose of organisms by the intravenous route. Details of this technique will be published fully elsewhere.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    (a) Rowley, D., Lancet, i, 232 (1955). (b) Landy, M., and Pillemer, L., J. Exp. Med., 104, 383 (1956). (c) Dubos, R. J., and Schaedler, R. W., ibid., 104, 53 (1956). (d) Howard, J. G., Rowley, D., and Wardlaw, A. C., Nature, 179, 314 (1957).

  2. 2

    Böhme, D., and Dubos, R. J., J. Exp. Med., 107, 532 (1958).

  3. 3

    Stuart, A. E., Biozzi, G., Stiffel, C., Halpern, B. N., and Mouton, D., Brit. J. Exp. Path. (in the press).

  4. 4

    Miles, A. A., and Misra, S. S., J. Hyg. (Camb.), 38, 732 (1938).

  5. 5

    Halpern, B. N., Biozzi, G., Mene, G., and Benacerraf, B., Ann. Inst. Bact., 80, 582 (1951).

  6. 6

    Benacerraf, B., Sebestyen, M. S., and Schlossmann, S., J. Exp. Med., 110, 27 (1959).

Download references

Author information

Affiliations

  1. Departments of Bacteriology and Pathology, University of Edinburgh

    • G. N. COOPER
    •  & A. E. STUART

Authors

  1. Search for G. N. COOPER in:

  2. Search for A. E. STUART in:

About this article

Publication history

Issue Date

DOI

https://doi.org/10.1038/191295a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.