Letter | Published:

A Spray Method for the Simultaneous Detection of Iodide and Iodinated Amino-Acids on Filter Paper Chromatograms

Naturevolume 191pages275276 (1961) | Download Citation



FOR Chromatographic identification of iodide and iodinated protein derivatives, it is often necessary to locate inorganic and organic iodide tracers on the same chromatogram. In paper chromatography a number of methods have been used for the detection of iodide. These include spraying with silver nitrate1 ; potassium chlorate, acid and starch2 ; 1 per cent aqueous palladium chloride3 ; a mixture of ferric nitrate and hydrogen peroxide4 ; the ceric-arsenite reagent5; and a mixture of FeCl3.6H2O, K3Fe(CN)6 and NaAsO2 (FFCA reaction)6. Most of these tests for iodide interfere with those commonly used for the identification of iodinated amino-acids, namely, ninhydrin7, diazotized sulphanilic acid3, or its diethyl amide8. The ceric-arsenite and the FFCA reagents are the most sensitive (0.01 µm. I) and can moreover be used for the detection of inorganic iodide, together with iodinated amino-acids on the same chromatogram6,9. The FFCA reagent, however, is not specific for iodine but also gives positive reactions with certain reducing substances6. The ceric-arsenite reaction has several disadvantages and is generally not used in the spraying technique9.

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    Gross, J., and Leblond, C. P., Endocrinol., 48, 714 (1951).

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    Block, R. J., Durrum, E. L., and Zweig, G., A Manual of Chromatography and Paper Electrophoresis, 319 (Acad. Press, Inc., New York, 1955).

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    Bowden, C. H., MacClagan, N. F., and Wilkinson, J. H., Biochem. J., 59, 93 (1955).

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    Smith, I., Chromatographic and Electrophoretic Techniques, 1, 172 (William Heinemann Medical Books, Ltd., London, 1960).

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  1. Department of Physiology, University of Cape Town

    • A. VAN ZYL
    •  & M. BHAGA


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