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A Spray Method for the Simultaneous Detection of Iodide and Iodinated Amino-Acids on Filter Paper Chromatograms

Abstract

FOR Chromatographic identification of iodide and iodinated protein derivatives, it is often necessary to locate inorganic and organic iodide tracers on the same chromatogram. In paper chromatography a number of methods have been used for the detection of iodide. These include spraying with silver nitrate1 ; potassium chlorate, acid and starch2 ; 1 per cent aqueous palladium chloride3 ; a mixture of ferric nitrate and hydrogen peroxide4 ; the ceric-arsenite reagent5; and a mixture of FeCl3.6H2O, K3Fe(CN)6 and NaAsO2 (FFCA reaction)6. Most of these tests for iodide interfere with those commonly used for the identification of iodinated amino-acids, namely, ninhydrin7, diazotized sulphanilic acid3, or its diethyl amide8. The ceric-arsenite and the FFCA reagents are the most sensitive (0.01 µm. I) and can moreover be used for the detection of inorganic iodide, together with iodinated amino-acids on the same chromatogram6,9. The FFCA reagent, however, is not specific for iodine but also gives positive reactions with certain reducing substances6. The ceric-arsenite reaction has several disadvantages and is generally not used in the spraying technique9.

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VAN ZYL, A., BHAGA, M. A Spray Method for the Simultaneous Detection of Iodide and Iodinated Amino-Acids on Filter Paper Chromatograms. Nature 191, 275–276 (1961). https://doi.org/10.1038/191275a0

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