Abstract
FOR Chromatographic identification of iodide and iodinated protein derivatives, it is often necessary to locate inorganic and organic iodide tracers on the same chromatogram. In paper chromatography a number of methods have been used for the detection of iodide. These include spraying with silver nitrate1 ; potassium chlorate, acid and starch2 ; 1 per cent aqueous palladium chloride3 ; a mixture of ferric nitrate and hydrogen peroxide4 ; the ceric-arsenite reagent5; and a mixture of FeCl3.6H2O, K3Fe(CN)6 and NaAsO2 (FFCA reaction)6. Most of these tests for iodide interfere with those commonly used for the identification of iodinated amino-acids, namely, ninhydrin7, diazotized sulphanilic acid3, or its diethyl amide8. The ceric-arsenite and the FFCA reagents are the most sensitive (0.01 µm. I−) and can moreover be used for the detection of inorganic iodide, together with iodinated amino-acids on the same chromatogram6,9. The FFCA reagent, however, is not specific for iodine but also gives positive reactions with certain reducing substances6. The ceric-arsenite reaction has several disadvantages and is generally not used in the spraying technique9.
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VAN ZYL, A., BHAGA, M. A Spray Method for the Simultaneous Detection of Iodide and Iodinated Amino-Acids on Filter Paper Chromatograms. Nature 191, 275–276 (1961). https://doi.org/10.1038/191275a0
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DOI: https://doi.org/10.1038/191275a0
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