Letter | Published:

Immunofluorescence of Pleuropneumonia-like Organisms isolated from Tissue Cell Cultures

Naturevolume 191pages190191 (1961) | Download Citation



IT has been established that continuous tissue cell cultures are commonly contaminated with pleuropneumonia-like organisms and/or L-forms of bacteria1–5. These results have encouraged investigators to search for reasonably simple methods for the detection of these agents, especially in relation to studies for their elimination. Of methods proposed for their detection, cultivation and isolation have proved most successful6. With the advent of the fluorescent antibody technique as a rapid method for the identification of micro-organisms, it seemed to us that this procedure might be useful as a means of detecting contamination of pleuropneumonia-like organisms in tissue cell cultures. The feasibility of this technique is presented here.

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  1. Armed Forces Institute of Pathology, Washington, D.C.

    • W. F. MALIZIA
  2. Laboratory of Bacterial Products, National Institutes of Health, Bethesda, Maryland

    • M. F. BARILE
    •  & D. B. RIGGS


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