Letter | Published:

Immunofluorescence of Pleuropneumonia-like Organisms isolated from Tissue Cell Cultures

Naturevolume 191pages190191 (1961) | Download Citation

Subjects

Abstract

IT has been established that continuous tissue cell cultures are commonly contaminated with pleuropneumonia-like organisms and/or L-forms of bacteria1–5. These results have encouraged investigators to search for reasonably simple methods for the detection of these agents, especially in relation to studies for their elimination. Of methods proposed for their detection, cultivation and isolation have proved most successful6. With the advent of the fluorescent antibody technique as a rapid method for the identification of micro-organisms, it seemed to us that this procedure might be useful as a means of detecting contamination of pleuropneumonia-like organisms in tissue cell cultures. The feasibility of this technique is presented here.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Robinson, L. R., Wichelhausen, R. H., and Roizman, B., Science, 124, 1147 (1956).

  2. 2

    Collier, L. H., Nature, 180, 757 (1957).

  3. 3

    Rothblat, G. H., and Morton, H. E., Bact. Proc., 73 (1958).

  4. 4

    Hayflick, L., and Stinebring, W. R., Ann. New York Acad. Sci., 79, 433 (1960).

  5. 5

    Macpherson, I. A., and Allner, K., Nature, 186, 992 (1960).

  6. 6

    Barile, M. F., Yaguchi, R., and Eveland, W. C., Amer. J. Clin. Path., 30, 171 (1958).

  7. 7

    Marshall, J. D., Eveland, W. C., and Smith, C. W., Proc. Soc. Exp. Biol. Med., 98, 898 (1958).

  8. 8

    Smith, C. W., Marshall, J. D., and Eveland, W. C., Proc. Soc. Exp. Biol. Med., 102, 179 (1959).

  9. 9

    Holmgren, N. B., and Campbell, jun., W. E., J. Bact., 79, 869 (1960).

Download references

Author information

Affiliations

  1. Armed Forces Institute of Pathology, Washington, D.C.

    • W. F. MALIZIA
  2. Laboratory of Bacterial Products, National Institutes of Health, Bethesda, Maryland

    • M. F. BARILE
    •  & D. B. RIGGS

Authors

  1. Search for W. F. MALIZIA in:

  2. Search for M. F. BARILE in:

  3. Search for D. B. RIGGS in:

About this article

Publication history

Issue Date

DOI

https://doi.org/10.1038/191190a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.