Letter | Published:

Simultaneous Determination of Dextran and Glucose in Serum

Naturevolume 191pages173174 (1961) | Download Citation

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Abstract

IN the course of an investigation on the mechanism of ‘anaphylactoid’ reaction in rats, the need was felt for an accurate and simple procedure for the simultaneous determination of dextran and glucose in small amounts of serum. For this purpose the following method was developed. It utilizes the o-toluidin – glucose coupling reaction1, producing a green colour suitable for colorimetric measurement. Dextran is precipitated from the serum by the o-toluidin – acetic acid reagent followed by hydrolysis and the colour reaction. Serum glucose is directly determined from the supernatant layer of the dextran precipitate.

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References

  1. 1

    Hultman, E., Nature, 183, 108 (1959).

  2. 2

    Dimler, R. J., Davis, H. A., Gill, G. J., and Rist, C. E., Anal. Chem., 26, 1142 (1954).

  3. 3

    Földes, J., Kisérl. Orvostud., 11, 1 (1959).

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Affiliations

  1. Institute of Pathophysiology, Medical University of Szeged

    • A. RÉDEI
    •  & S. NAGY

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https://doi.org/10.1038/191173a0

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