Abstract
RECENT biochemical studies on succinic dehydrogenase in livers of rats fed with the liver carcinogen p-dimethylaminoazobenzene (DAB) for periods of up to 150 days have confirmed the well-established phenomenon that the level of this enzyme generally falls as feeding progresses (Table 1). Samples of tissue from each liver lobe were taken for histological and histochemical investigations, and the remainder was homogenized. Succinic dehydrogenase was estimated to within an accuracy of ± 10 per cent by the anaerobic methylene blue technique, basing the QMB values on dry weight. For histochemical purposes the tissue was frozen1, stored for not longer than a week, and cut at − 20° C. in a refrigerated microtome. Succinic dehydrogenase was detected higtochemically by incubating frozen sections in a neotetrazolium-succinate buffer system2; in addition, the periodic acid–Schiff method3 was used to stain glycogen, while phospholipid was stained by the acid hæmatin method4.
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JONES, G., BITENSKY, L., CHAYEN, J. et al. Tissue Dilution Artefact; a Re-interpretation of Variations in Levels of Succinic Dehydrogenase during Chemical Carcinogenesis. Nature 191, 1203 (1961). https://doi.org/10.1038/1911203a0
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DOI: https://doi.org/10.1038/1911203a0
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