Letter | Published:

Simplified Procedure for removing Non-specific Staining Components from Fluorescein-labelled Conjugates

Naturevolume 191pages7071 (1961) | Download Citation

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Abstract

Coons and Kaplan1 in their description of the fluorescent antibody technique using antisera coupled with fluorescein isocyanate found that the nonspecific staining which occurred when such conjugates were applied to tissue sections could be minimized by dialysing the conjugates against 0.01 M phosphate buffer solution pH 7.0 for several days, followed by treatment with liver powder.

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References

  1. 1

    Coons, A. H., and Kaplan, M. H., J. Exp. Med., 91, 1 (1950).

  2. 2

    Marshall, J. D., Eveland, W. C., and Smith, C. W., Proc. Soc. Exp. Biol., 98, 898 (1958).

  3. 3

    Glynn, L. E., Holborow, E. J., and Johnson, G. D., Lancet, ii, 1083 (1957).

  4. 4

    Holborow, E. J., Weir, D. M., and Johnson, G. D., Brit. Med. J., ii, 732 (1957).

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Affiliations

  1. Rheumatism Research Unit (Medical Research Council), Canadian Red Cross Memorial Hospital, Taplow, Maidenhead

    • G. D. JOHNSON

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https://doi.org/10.1038/191070a0

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