Letter | Published:

Simplified Procedure for removing Non-specific Staining Components from Fluorescein-labelled Conjugates

Naturevolume 191pages7071 (1961) | Download Citation



Coons and Kaplan1 in their description of the fluorescent antibody technique using antisera coupled with fluorescein isocyanate found that the nonspecific staining which occurred when such conjugates were applied to tissue sections could be minimized by dialysing the conjugates against 0.01 M phosphate buffer solution pH 7.0 for several days, followed by treatment with liver powder.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Coons, A. H., and Kaplan, M. H., J. Exp. Med., 91, 1 (1950).

  2. 2

    Marshall, J. D., Eveland, W. C., and Smith, C. W., Proc. Soc. Exp. Biol., 98, 898 (1958).

  3. 3

    Glynn, L. E., Holborow, E. J., and Johnson, G. D., Lancet, ii, 1083 (1957).

  4. 4

    Holborow, E. J., Weir, D. M., and Johnson, G. D., Brit. Med. J., ii, 732 (1957).

Download references

Author information


  1. Rheumatism Research Unit (Medical Research Council), Canadian Red Cross Memorial Hospital, Taplow, Maidenhead

    • G. D. JOHNSON


  1. Search for G. D. JOHNSON in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.