Nucleic Acid Content of Haploid and Diploid Cells of Protomyces inundatus

Abstract

Protomyces is a fungus of uncertain taxonomic position and its life-history is incompletely known. In particular, previous investigators1,2 have been unable to determine satisfactorily the position of meiosis. In the course of a taxonomic investigation into the genus Protomyces, a number of standard staining techniques was employed in an attempt to demonstrate meiosis, but up to now none has proved satisfactory. In view of the recent demonstration of a linear relationship between deoxyribonucleic acid (DNA) content and ploidy in a polyploid series of Saccharomyces 3, we decided to use DNA estimation as the basis of a method for distinguishing between haploid and diploid stages in Protomyces.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Von Buren, G., Beitr. z. Krypt. d. Schweiz., 5, i 1 (1915); iii, 1 (1915–1923).

  2. 2

    Dangeard, P. A., Le Botaniste, 9e Serie, 263 (1903–1906).

  3. 3

    Ogur, M., Minckler, S., Lindegren, G., and Lindegren, C. C., Arch. Biochem. and Biophys., 40, 175 (1952).

  4. 4

    Lilly, V. G., and Barnett, H. L., Physiology of the Fungi (McGraw-Hill, 1951).

  5. 5

    Ebel, J. P., Bull. Soc. Chim. Biol., Paris, 34, 491 (1952).

  6. 6

    Paech, K., and Tracey, M. V., Modern Methods of Plant Analysis, 4, 291 (1955).

  7. 7

    Hatch, W. R., Ann. Bot., N.S., 2, 583 (1938).

  8. 8

    Ramanathan, K. R., Ann. Bot., N.S., 3, 375 (1939).

  9. 9

    Heagy, F. C., and Roper, J. A., Nature, 170, 713 (1952).

  10. 10

    Lawton, E., Amer. J. Bot., 19, 303 (1932).

  11. 11

    Thaysen, A. C., and Morris, M., Nature, 152, 523 (1943).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

VALADON, L., MANNERS, J. & MYERS, A. Nucleic Acid Content of Haploid and Diploid Cells of Protomyces inundatus . Nature 190, 836–837 (1961). https://doi.org/10.1038/190836a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.