Nucleic Acid Content of Haploid and Diploid Cells of Protomyces inundatus


Protomyces is a fungus of uncertain taxonomic position and its life-history is incompletely known. In particular, previous investigators1,2 have been unable to determine satisfactorily the position of meiosis. In the course of a taxonomic investigation into the genus Protomyces, a number of standard staining techniques was employed in an attempt to demonstrate meiosis, but up to now none has proved satisfactory. In view of the recent demonstration of a linear relationship between deoxyribonucleic acid (DNA) content and ploidy in a polyploid series of Saccharomyces 3, we decided to use DNA estimation as the basis of a method for distinguishing between haploid and diploid stages in Protomyces.

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VALADON, L., MANNERS, J. & MYERS, A. Nucleic Acid Content of Haploid and Diploid Cells of Protomyces inundatus . Nature 190, 836–837 (1961).

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