Spontaneous Potentials from Explants of Chick Cerebral Hemisphere (Telencephalon) in Tissue Culture

Abstract

USING techniques and supernatant similar to those used to demonstrate spontaneous potentials from explants of chick embryo cerebellum in tissue culture in Kahn tubes¹, spontaneous potentials have been demonstrated in explants from the telencephalon of 11-day chick embryo brain. The explants were thin slices of the full thickness of the lateral aspect of the telencephalon. The control used was dead chick cerebellum tissue (explanted into a Kahn tube in the same manner as the telencephalic tissue and then killed by heat). The Kahn tube containing the control tissue was placed side by side with the living culture material in the same incubator with identical amplification and recording apparatus. The living brain tissue gave the same form, and magnitude of potentials on every recording channel which was tried; whereas the control tissues gave no potentials on any channel.