Abstract
HUMAN serum reacts with hæmolysates of human erythrocytes to form a precipitate ; this occurs even with the isologous hæmolysate. When the hæmolysate is added to serum in tubes at 37° C., precipitation continues for several days. In agar-gel, using a double diffusion technique, a well-marked precipitate band is formed (Fig. 1). Peetoom et al. 1 described a precipitate of this type in agar-gel, but only minimal precipitation in fluid media. In spite of antigenic differences between adult and fœtal hæmoglobins2, serum and hæmolysate from adult and umbilical cord blood form precipitates in agar-gel, which are qualitatively the same. Cross-precipitation also occurs between human and some animal sera and hæmolysates. As the reaction is of general occurrence, it causes false positive results in tests for auto-antibodies when the tissue extract used as antigen contains sufficient lysed erythrocytes.
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References
Peetoom, F., Rose, N., Ruddy, S., Micheli, A., and Grabar, P., Ann. Inst. Pasteur, 98, 252 (1960).
Recent Advances in Clin. Path., Series 3, 202 (Churchill, 1960).
Tuttle, A. H., Science, 121, 701 (1955).
Allison, A. C., and ap Rees, W., Brit. Med. J., ii, 1137 (1957).
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WILSON, J., JOBLING, D. Precipitation Reaction between Serum and Lysed Erythrocytes. Nature 190, 550 (1961). https://doi.org/10.1038/190550a0
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DOI: https://doi.org/10.1038/190550a0
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