Abstract
AN aqueous solution of nucleoprotein, concentration 12 mgm./ml., prepared from calf thymus by the method of Kay, Simmons and Bounce1, was irradiated with high-energy electrons to a dose of 4 × 106 rads. The dose was given in 4 min., and was assumed to be delivered to an oxygen-free solution, the initial 2 per cent of the dose using up all dissolved oxygen2. It was noted that immediately following irradiation a white suspension had been formed, and this afterwards collected at the bottom of the tube on standing. When the irradiated and control solutions were analysed it was found that in addition to producing a precipitate, irradiation had degraded amino-acids, purine and pyrimidine bases and deoxyribose sugar. Details of these degradations will be published elsewhere ; the present report is concerned with the nature of the precipitate.
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HEMS, G. A New Effect of Ionizing Radiation on Nucleoprotein. Nature 189, 849–851 (1961). https://doi.org/10.1038/189849a0
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DOI: https://doi.org/10.1038/189849a0
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