Action of Trypsin on Bence-Jones Protein Derivatives

Abstract

IT is well known that the proteolytic enzyme trypsin fails to hydrolyse native proteins, or hydrolyses them to only a very small extent. The denaturation of protein substrate is therefore a customary preliminary operation in all work involving the action of trypsin on proteins. We report here the effect of trypsin on some chemical derivatives of a pure de-ionized1 Bence-Jones protein isolated in large amounts from the urine of a single patient.

References

  1. 1

    Timasheff, S. N., Dintzis, H. M., Kirkwood, J. G., and Coleman, B. D., J. Amer. Chem. Soc., 79, 782 (1957).

    CAS  Article  Google Scholar 

  2. 2

    Harrington, W. F., and Schellman, J. A., C.R. Trav. Lab., Carlsberg, Sér. chim., 30, 21 (1956).

    CAS  Google Scholar 

  3. 3

    Bailey, J. L., and Cole, R. D., J. Biol. Chem., 234, 1733 (1959).

    CAS  PubMed  Google Scholar 

  4. 4

    White, jun., F. H., J. Biol. Chem., 235, 383 (1960).

    CAS  PubMed  Google Scholar 

  5. 5

    Stauff, J., and Duden, R., Biochem. Z., 331, 10 (1958).

    CAS  PubMed  Google Scholar 

  6. 6

    Sela, M., White, jun., F. H., and Anfinsen, C. B., Biochim. Biophys. Acta, 31, 417 (1959).

    CAS  Article  Google Scholar 

Download references

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FERRINI, U., ZITO, R. Action of Trypsin on Bence-Jones Protein Derivatives. Nature 189, 485 (1961). https://doi.org/10.1038/189485a0

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