IT has been, pointed out that mammalian sperm represents an ideal tissue for exploring, under well-defined conditions in vitro, the intricate biochemical and physiological mechanisms underlying the viability and senescence of living cells1. Efforts to move in this direction have been restricted by the necessity of having to maintain mammalian sperm in complex, undefined media and at temperatures which suppress metabolic activity.
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NORMAN, C., GOLDBERG, E., PORTERFIELID, I. et al. Prolonged Survival of Human Sperm in chemically defined Media at Room Temperatures. Nature 188, 760 (1960). https://doi.org/10.1038/188760a0
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