An Effect of Early Dilution on the Establishment of Lysogeny in Salmonella typhimurium

Abstract

IT is known that the frequency of lysogenic response in a culture of Salmonella typhimurium strain LT2, infected with the temperate bacterio-phage P22, can be influenced by the ratio of phage to bacterium (multiplicity of infection)1–3, and by modifying the metabolism of the infected cells soon after infection, for example, by the addition of chloramphenicol4. With a high multiplicity of infection a high frequency of lysogenic response is obtained. Using a low multiplicity of infection a low lysogenic response results. If these infected cells are exposed to chloramphenicol (25 µgm./ml.) 5 min. after infection and the chloramphenicol removed by dilution 15 min. later, then practically every cell infected becomes lysogenic.

References

  1. 1

    Boyd, J. S. K., J. Path. Bact., 63, 445 (1951).

  2. 2

    Lwoff, A., Kaplan, A. S., and Ritz, E., Ann. Inst. Pasteur, 86, 127 (1954).

  3. 3

    Levine, M., Virology, 3, 22 (1957).

  4. 4

    Bertani, L. E., Virology, 4, 53 (1957).

  5. 5

    Lanni, Y. T., Virology, 10, 501 (1960).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

MOSELEY, B., GORRILL, R. An Effect of Early Dilution on the Establishment of Lysogeny in Salmonella typhimurium . Nature 188, 755 (1960). https://doi.org/10.1038/188755a0

Download citation

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.