IT is known that the frequency of lysogenic response in a culture of Salmonella typhimurium strain LT2, infected with the temperate bacterio-phage P22, can be influenced by the ratio of phage to bacterium (multiplicity of infection)1–3, and by modifying the metabolism of the infected cells soon after infection, for example, by the addition of chloramphenicol4. With a high multiplicity of infection a high frequency of lysogenic response is obtained. Using a low multiplicity of infection a low lysogenic response results. If these infected cells are exposed to chloramphenicol (25 µgm./ml.) 5 min. after infection and the chloramphenicol removed by dilution 15 min. later, then practically every cell infected becomes lysogenic.
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MOSELEY, B., GORRILL, R. An Effect of Early Dilution on the Establishment of Lysogeny in Salmonella typhimurium . Nature 188, 755 (1960). https://doi.org/10.1038/188755a0