Crystallization of the Complex of D-Amino-Acid Oxidase and Benzoate

Abstract

WE have been, investigating D-amino-acid oxidase apo-protein, flavin adenine dinucleotide and the substrate with reference to complex formation1,2. In the previous communication1, it was reported that the addition of the apo-protein to flavin adenine dinucleotide causes a shift and decrease of the absorption peak at 450 mµ of the latter. It was also found that the co-existence of benzoate, a substrate-substitute, and the apo-protein causes further marked changes in the absorption spectrum of the flavin, adenine dinucleotide. Two important changes were especially noted : (1) the intensity of the absorption at 450 mµ is further decreased and that at 485 mµ is increased to form a shoulder, and (2) a new absorption peak appears at 465 mµ. These results indicate that both the apo-protein and the benzoate combine with two different binding sites of the isoalloxazine nucleus of flavin adenine dinucleotide. On the other hand, the fact that benzoate competes with the substrate, D-amino-acid3,4, indicates combination between benzoate and the apo-protein. Hence it may be considered that the apo-protein, flavin adenine dinucleotide and benzoate combine to form a tight complex5.

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References

  1. 1

    Yagi, K., Ozawa, T., and Harada, M., Nature 184, 1939 (1959).

  2. 2

    Yagi, K., and Ozawa, T., Biochim. Biophys. Acta, 42, 381 (1960).

  3. 3

    Klein, J. R., J. Biol. Chem., 205, 725 (1953).

  4. 4

    Yagi, K., Ozawa, T., and Okada, K., Biochim. Biophys. Acta, 35, 102 (1959).

  5. 5

    Yagi, K., Ozawa, T., and Harada, M., Symposium on Enzyme Chemistry (Japan), 11, 37 (1959).

  6. 6

    Yagi, K., Okuda, J., Ozawa, T., and Okada, K., Biochim. Biophys. Acta, 34, 372 (1959).

  7. 7

    Kubo, H., Yamano, T., Iwatsubo, M., Watari, H., Soyama, T., Shiraishi, J., Sawada, S., and Kawashima, N., Bull. Soc. Chim. Biol., 90, 431 (1958).

  8. 8

    Massey, V., and Palmer, C., Biochem. J., 74, 40P (1960).

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YAGI, K., OZAWA, T. & HARADA, M. Crystallization of the Complex of D-Amino-Acid Oxidase and Benzoate. Nature 188, 745–746 (1960). https://doi.org/10.1038/188745a0

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