Letter | Published:

Induced Fluorescence with Acridine Orange, after Osmium Fixation

Naturevolume 187pages964965 (1960) | Download Citation



THE differential staining of nucleic acids with fluorochromes1 has provided a useful tool for the study of virus-infected tissue cells2 ; but its value has been limited by the fact that the common fixatives which permit the use of this method also tend to produce artefacts, such as the gross nuclear vesiculation observed in cells infected with some types of adenovirus2, but not observed by phase-contrast examination of the living infected cell3.

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    Armstrong, J. A., Exp. Cell Res., 11, 640 (1956).

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    Anderson, E. S., Armstrong, J. A., and Niven, J. S. F., Ninth Symp. Soc. Gen. Microbiol., 224 (1959).

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    Armstrong, J. A., and Hopper, P. K., Exp. Cell Res., 16, 584 (1959).

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    Porter, K. R., Claude, A., and Fullam, E. F., J. Exp. Med., 81, 233 (1945).

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    Palade, G. E., J. Exp. Med., 95, 285 (1952).

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    Westwood, J. C. N., MacPherson, I. A., and Titmuss, D. H. J., Brit. J. Exp. Path., 38, 138 (1957).

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  1. Microbiological Research Establishment, Porton, Wilts

    • W. J. RANDLES


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