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A Method for the Isolation of Plant Protoplasts and Vacuoles

Nature volume 187, pages 962–963 (1960)Cite this article

Abstract

THE isolation of bacterial1 and fungal protoplasts2,3 following the use of enzymes digesting the cell wall suggested that protoplasts might be obtained from plant cells by treatment with cellulase. Root tips of tomato seedlings (Lycopersicum esculentum Mill. var. Sutton's ‘Best of All’) were selected for investigation, and a fungal cellulase obtained from Myrothecium verrucaria 4 (kindly supplied by Dr. D. R. Whitaker, National Research Council, Canada) was employed.

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References

  1. Weibull, C., “Ann Rev. Microbiol.”, 12, 1 (1958).

    Article  CAS  Google Scholar 

  2. Eddy, A. A., and Williamson, D. H., Nature, 179, 1252 (1957).

    Article  ADS  Google Scholar 

  3. Bachmann, B. J., and Bonner, D. M., J. Bact., 78, 550 (1959).

    CAS  PubMed  Google Scholar 

  4. Whitaker, D. R., Arch. Biochem. Biophys., 43, 253 (1953).

    Article  CAS  Google Scholar 

  5. Preston, R. D., “The Molecular Architecture of Plant Cell Walls” (Chapman and Hall, London, 1952).

    Book  Google Scholar 

  6. Meeuse, A. D. J., Bot. Rev., 7, 249 (1941).

    Article  Google Scholar 

  7. Brenner, S., Dark, F. A., Gerhardt, P., Jeynes, M. H., Kandler, O., Kellenberger, E., Klieneberger-Nobel, E., McQuillen, K., Rubio-Huertos, M., Salton, M. J. R., Strange, R. E., Tomcsik, J., and Weibull, C., Nature, 181, 1713 (1958).

    Article  ADS  Google Scholar 

  8. Bennet-Clark, T. A., in “Plant Physiology”, edit. by Steward, F. C., 2 (Academic Press, 1959).

    Google Scholar 

  9. Cocking, E. C., Biochem. J. (in the press).

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Authors and Affiliations

  1. Department of Botany, University of Nottingham,

    E. C. COCKING

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  1. E. C. COCKING
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COCKING, E. A Method for the Isolation of Plant Protoplasts and Vacuoles. Nature 187, 962–963 (1960). https://doi.org/10.1038/187962a0

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