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A Method for the Isolation of Plant Protoplasts and Vacuoles

Naturevolume 187pages962963 (1960) | Download Citation



THE isolation of bacterial1 and fungal protoplasts2,3 following the use of enzymes digesting the cell wall suggested that protoplasts might be obtained from plant cells by treatment with cellulase. Root tips of tomato seedlings (Lycopersicum esculentum Mill. var. Sutton's ‘Best of All’) were selected for investigation, and a fungal cellulase obtained from Myrothecium verrucaria 4 (kindly supplied by Dr. D. R. Whitaker, National Research Council, Canada) was employed.

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  1. 1

    Weibull, C., “Ann Rev. Microbiol.”, 12, 1 (1958).

  2. 2

    Eddy, A. A., and Williamson, D. H., Nature, 179, 1252 (1957).

  3. 3

    Bachmann, B. J., and Bonner, D. M., J. Bact., 78, 550 (1959).

  4. 4

    Whitaker, D. R., Arch. Biochem. Biophys., 43, 253 (1953).

  5. 5

    Preston, R. D., “The Molecular Architecture of Plant Cell Walls” (Chapman and Hall, London, 1952).

  6. 6

    Meeuse, A. D. J., Bot. Rev., 7, 249 (1941).

  7. 7

    Brenner, S., Dark, F. A., Gerhardt, P., Jeynes, M. H., Kandler, O., Kellenberger, E., Klieneberger-Nobel, E., McQuillen, K., Rubio-Huertos, M., Salton, M. J. R., Strange, R. E., Tomcsik, J., and Weibull, C., Nature, 181, 1713 (1958).

  8. 8

    Bennet-Clark, T. A., in “Plant Physiology”, edit. by Steward, F. C., 2 (Academic Press, 1959).

  9. 9

    Cocking, E. C., Biochem. J. (in the press).

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  1. Department of Botany, University of Nottingham

    • E. C. COCKING


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