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Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose

Naturevolume 187pages11271128 (1960) | Download Citation



THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations1. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day2.

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  1. 1

    Milstone, J. H., Proc. Soc. Exp. Biol. and Med., 103, 361 (1960).

  2. 2

    Sober, H. A., Gutter, F. J., Wyckoff, M. M., and Peterson, E. A., J. Amer. Chem. Soc., 78, 756 (1956).

  3. 3

    Milstone, J. H., J. Gen. Physiol., 42, 665 (1959).

  4. 4

    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).

  5. 5

    Mellanby, J., and Pratt, C. L. G., Proc. Roy. Soc., B, 128, 201 (1939).

  6. 6

    Sherry, S., and Troll, W., J. Biol. Chem., 208, 95 (1954).

  7. 7

    Milstone, J. H., J. Gen. Physiol., 38, 757 (1955).

  8. 8

    Macfarlane, R. G., J. Physiol., 106, 104 (1947).

  9. 9

    Arscott, P. M., Koppel, J. L., and Olwin, J. H., Nature, 183, 753 (1959).

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  1. Department of Pathology, Yale University School of Medicine, New Haven, Connecticut

    • J. H. MILSTONE


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